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Medical Laboratory Technician (MLT)

Medical Laboratory Technician (MLT)

This checklist was electronically signed on September 12, 2026

Instructions: This checklist is meant to serve as a general guideline for our client facilities as to the level of your skills within your nursing specialty. Please use the scale below to describe your experience/expertise in each area listed below.

Proficiency Scale:

1 = No Experience

2 = Need Training

3 = Able to perform with supervision

4 = Able to perform independently

PATIENT RIGHTS

Communicate and obtain information while respecting patient rights, privacy, and confidentiality of information in accordance with the Health Insurance Portability and Accountability Act of 1996 (HIPAA)
Involve the patient and family and respect their role in determining the nature of care to be provided
Meet patient's and families' needs regarding communication, including interpreter services
Provide accurate information to patients and families in a timely manner

COMMUNICATION

Communicate with patients and their companions in a courteous manner
Understand the chain of communication, who to contact, and when
Listen actively to patient concerns and address them appropriately.
Cultural Sensitivity and Language Skills

GENERAL INFECTION CONTROL MEASURES

Aseptic Technique
Standard Precaution
Universal Precaution

TRANSMISSION-BASED PROTOCOL

Airborne PrecautionAirborne Precaution
Contact Precaution
Droplet Precaution
Reverse Precaution

GENERAL LABORATORY TESTING

Type of Sample for Test Required
Method for Test Requested
Reagents for Test
Media for Test
Control for Test
Instrument to Perform Test
Routine Procedure to Verify Result
Special Procedure to Verify the Result

GENERAL LAB TESTING - PREPARE APPROPRIATE

Blood Products
Controls
Instruments
Media
Reagents
Samples
Accurately Calculate Results of Testing

INSTRUMENTATION & TESTING METHODS

Blood Bank: Manual Gel Testing using Ortho
Blood Gases: GEM 4000
Chemistry: Ortho Vitros 7600 (2 analyzers)
Coagulation: Siemens CS-2500 (with backup Siemens CS-5100 & no backup available)
Drug screens: MedTox
Hematology: Sysmex XN-1000 (primary) and XN-550 (backup)
Microbiology: blood culture gram stains/stat gram stains (with & without plating)
Molecular: Cepheid
Urinalysis: Arkray and Siemens Clinitek Status Plus
Various Kits: Pregnancy, Mono, HIV Rapid, Strep A, etc.

EVALUATE LABORATORY DATA

Recognize Related Disease States
Verify Test Results for Reporting
Recognize Inconsistent Results
Recognize Sources of Error
Check for Common Technical Problems
Take Corrective Action
Determine Instrument Adjustments
Assess Accuracy of Test Results
Recognize / Report Abnormal Results
Recognize Need for Additional Testing

GENERAL LABORATORY PRACTICE

Glucose Tolerance Testing
Glucometer Use

BLOOD BANK

ABO Testing
Antibody Identification
Antibody Screen
Antibody Titer
Blood Irradiation
Cell Count
Cell Packing
Cell Washing
Compatibility Testing
Coombs Test Direct
Coombs Test Indirect
Cord Blood
Cross Match
Cryoprecipitate
Emergency Release
Freezing Blood
Fresh Frozen Plasma (FFP)
Immediate Spin Cross Match
Issuing Blood Components
Patient Identification
Plasmapheresis
Plateletpheresis
Platelets
RBC's
Rh IgG
RH Testing
Rhogam
Thawing Procedures for Fresh Frozen Plasma (FFP)
Thawing Procedures for Platelets
Transfusion Adverse Reaction
Transfusion Practices/Indications

CHEMISTRY

Acetone Analysis (Serum and Urine)
Ammonia
Basic Metabolic Panel (BMP)
Blood Alcohol Level
Blood Gases
C-Reactive Protein (CRP) Test
Cerebrospinal Fluid Protein
Comprehensive Metabolic Panel (CMP)
Drug Screening
Electrophoresis
Gas Chromatography
Immunoassay Chemistry
Immunoelectrophoresis
Ion-Selective Electrodes Procedures
Iron and Total Iron-Binding Capacity (TIBC)
Osmolality
Therapeutic Drug Levels
Thin Layer Chromatography
Toxicology
Tumor Markers

COAGULATION

Bleeding Times
Circulating Anticoagulants
Fibrinogen
PT, PTT, INR
Thrombin Time

HEMATOLOGY

Bone Marrow Preparation
CBC (Count, Morph, Differential)
Cerebrospinal Fluid (CSF)
Erythrocyte Sedimentation Rate (ESR)
Morphology (RBC, WBC, Differential, Platelet)
Red Blood Cells and Indices
Reticulocyte
Sickle Cell Prep
Tests for Hemoglobin Defects
White Blood Cell Count

HISTOLOGY

Assist with Frozen Sectioning
Assist with Gross Sectioning
Bone Marrow Biopsy and Slides
Collection of Tissue Specimen
Coverslipping
Crypto-Spin Preps
Decalcify Bone and Hand Tissue
Disposal of Wet Tissues
Embed Tissue
Filing of Slides and Blocks
Grossing Small Specimen
Immuno Stains
Microtome and Disposable Blades
MOHS Sectioning
Preparation of Stains/Reagents
Principle of Hematoxylin and Eosin Stain
Proper Labeling of Reagents
Running of the Tissue Processor
Special Stain Techniques
Storage Time of Wet Tissues
Universal Histology Precautions

IMMUNOLOGY/SEROLOGY

Antinuclear Antibody (ANA)
Antistreptolysin O (ASO) Titer
Autoimmunity
C3,C4, Total ComplementC3,C4, Total Complement
Cellular and Humoral
Cold Agglutinins
Cytomegalovirus (CMV)
DNA Probe Technology
ELISA Testing
Febrile Agglutinins
Flow Cytometry
Fluorescent Treponemal Antibody Absorption (FTA-ABS)
H. Pylori
Hemagglutination Inhibition
Hepatitis
HIV
Hormone Testing
Influenza Screen A and B
LE Cell Prep (Lupus)
Meningitis Screen
MMR Serology
Monospot
Mycoplasma
Nephelometry
Pertussis
Polymerase Chain Reaction (PCR) Technology
Pregnancy Testing/HCG
Radial Immuno Diffusion
Rapid Plasma Reagin (RPR) Test
Rapid Strep
Respiratory Syncytial Virus (RSV)
Rheumatoid Factor
Rubella Serology
T and B Cell Studies
TORCH Studies
Western Blot Technology

MICROBIOLOGY

Acid Fast Stain
Aerobic Gram Positive Cocci
Anaerobes
C Difficile Assay
Chlamydia
CO2 Cultures
Culture Plating
Dark Field
Fungus
General Bacteriology
Gram- Negative Bacilli
Gram- Negative Cocci
Gram- Positive Bacilli
India Ink
KOH Prep
Malarial Exam
Mycobacteria Culture
Mycobacterium
Occult Blood
Organism Identification
Parasites
Prep and ID
Sensitivities (KB and MIC)
Viruses
Wet Mount

PHLEBOTOMY

Ability to Write Procedures
Anticoagulants - Knowledge of Tube Types and Uses
Arterial Blood Gases
Basic Safety Guidelines: Gloves and Goggles
Calculation for Solution Prep: Molarity and Molality
Communication Skills
Finger Stick
Heel Stick
M.L.A
Masks
Operation of Centrifuge
Pediatric
Pipetting
To Contain
To Deliver
Use of Balance (Weighing)
Venipuncture
Volumetric

URINALYSIS

24-Hour Urine Collection
Automated Urinalysis
Crystal Identification
Manual Urinalysis
Microscopic Urinalysis
Semen Analysis
Urine Drug Testing
Urine Pregnancy Test

ELECTRONIC DOCUMENTATION

Allscripts
Care360
Cerner (Oracle)
CPSI
eCLinicalWorks
Eclipsys
EPIC/Beaker
MACLAB
McKesson/Paragon
Meditech
PACS
Quadramed
Soarian
WellskyWellsky

AGE-SPECIFIC COMPETENCIES

Newborn/Neonate (birth-30 days)
Infant (30 days- 1 year)
Toddler (1-3 years)
Preschooler (3-5 years)
School-age children (5-12 years)
Adolescents (12-18 years)
Young adults (18- 39 years)
Middle adults (39- 64 years)
Older adults (64-79 years)
Elderly adults (80+ years)